Upstream Processing Lab

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    From cloning genes of interest to fermentation at various scales, our Upstream Processing Lab offers comprehensive services to kickstart your product development journey.

Services:

• Cloning gene of interest in high expression vectors.
• Identification of suitable host for the recombinant protein production.
• MCB/WCB preparation and characterization.
• High-throughput screening of clones.
• Media optimization to enhance protein yield.
• Small scale fermentation- Shake flask.
• Fermentation from 1L to 50L scale.
• Fermentation- Microbial,yeast and mammalian.
• Various level operations like biomass clarification, lysis etc.,

Utilizing multiple purification techniques including chromatography and dialysis, our Downstream Processing Lab ensures the isolation of pure recombinant proteins.

Services:

• Multiple protein purification techniques including Affinity, Ion Exchange, Gel filtration, HIC and Endotoxin removal Chromatography.
• Selection and optimization of purification resins.
• Purification of inclusion bodies.
• Dialysis/Buffer exchange using TFF.
• Method development for purification of tagged/native protein to transfer to a cGMP facility.

Characterizing efficacy, purity and impurities of analytes, our Bioanalytical Lab offers a range of assays.

Services:

1.Efficacy of analyte:

ELISA (Direct/Indirect) to determine Affinity, EC50, as well as to support PK/PD.

2.Charactrization of analyte:

HPLC (SEC,RP,IEX), QELISA, Bioassay, Native-PAGE, Western blotting and Relative stability.

3.Impurity analysis:

HCP, HCDNA, ET, Residual Antibiotic testing, Ligand leachate assay, Sterility assays.

4.Cell based assay:

Bioassay development and validation with various cell lines to determine IC50 as per ICH guidelines.